Anti-bacterial dynamic hydrogels prepared from O-carboxymethyl chitosan by dual imine bond crosslinking for biomedical applications

Imine dynamic hydrogels are synthesized via dual-imine bond crosslinking from O-carboxymethyl chitosan (CMCS) and a water soluble dynamer using ‘green’ approach. Three dynamers prepared through reaction of benzene-1,3,5-tricarbaldehyde di-amino Jeffamine with molar mass 500, 800 1900, respectively. Hydrogels, namely H500, H800 H1900 then obtained by mixing CMCS aqueous solutions. FT-IR confirms the formation imine bonding. presents larger pore size higher storage modulus as compared to H500 due linker. The exhibit pH sensitive swelling behavior electrostatic attraction or repulsion in range 3 10. highest ratio is at 8, reaching 7500% for H800. Self-healing evidenced rheological measurements alternatively applied low high strains, macroscopic approach re-integration injected filaments. Furthermore, membrane exhibits outstanding antibacterial activity against an E. coli suspension 108 CFU mL−1. Therefore, present rheological, swelling, self-healing properties, most promising healthcare material wound dressing, drug delivery tissue engineering.